The invention provides a method allowing reverse differentiation of human somatic cells for generation of autologous pancreatic stem cells and autologous pancreas islet. The method is as follows: culture solutions containing extractives of different plants and different protein components are used to culture human somatic cells step by step, which enables the somatic cells to generate autologous pancreatic stem cells through reverse differentiation, and autologous pancreas islet is generated through further culture. The method provided in the invention enables the first generation of autologous pancreatic stem cells and autologous pancreas islet at a magnitude order of tens of billions to be generated within two to three weeks. The plant and protein-induced human autologous pancreatic stem cells have similar cell specific phenotypes and a plurality of cell differentiation capability as those of human natural pancreatic stem cells; the autologous pancreatic stem cells and autologous pancreas islet cells have immense potential both in the application field of treatment of pancreatic diseases like insulin deficiency type diabetes and in the engineering field of autologous pancreatic tissue; the invention is also applicable to establishment of novel autologous pancreatic stem cell libraries and long-term permanent preservation of autologous pancreatic stem cells.

Title
Method and kit allowing reverse differentiation of human somatic cells for generation of autologous pancreatic stem cells and autologous pancreas islet, and application thereof
Application Number
201010289087
Publication Number
102399740A
Application Date
September 19, 2010
Publication Date
April 4, 2012
Inventor
Lin Xiongbin
Agent
liu danni
Assignee
Lin Xiongbin
IPC
A61P3/10
A61P1/18
A61P13/12
A61P1/16
A61P25/00
A61P5/00
A61K35/39
C12N5/074
C12N5/077
C12N5/073
C12N5/078
C12N5/071